|Title:||Microbial Ultrastructure: The Use of the Electron Microscope (Technical Series - Society for Applied Bacteriology ; No. 10)|
|Format:||doc azw mbr lrf|
|ePUB size:||1920 kb|
|FB2 size:||1123 kb|
|DJVU size:||1971 kb|
|Publisher:||Academic Pr (June 1, 1977)|
Society for Applied Bacteriology technical series ; no. 10. General Note: Based on contributions made to the 1974 autumn demonstration meeting of the Society for Applied Bacteriology. On this site it is impossible to download the book, read the book online or get the contents of a book. The administration of the site is not responsible for the content of the site. The data of catalog based on open source database. All rights are reserved by their owners.
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Microbial Ultrastructure: The Use of the Electron Microscope (Technical Series - Society for Applied Bacteriology ; No. 10). Author : RoyFuller,D.
Overview of the transmission electron microscope. The resolution of a compound microscope is limited to half of the wavelength of the radiation used for imaging. This limits the light microscope to a maximum resolution of 200 nm, when using visible light with a wavelength of 400-. Another difficulty arises from the nature of the electron beam that is invisible to the human eye. Hence, auxiliary devices that convert the electron image into a visible light image are needed. For a well corrected optics, the resolution limit of the microscope is dependent on the wavelength of the radiation beam. The use of specific antibodies also allows a more precise identification of the virus particles. If this is required, the viruses adsorbed to the membrane-covered grid or thin sections of virus infected tissues, can be treated with gold-labelled antibody.
The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr’s resin is combined with acetone and LR-White resin is mixed with ethanol. Paula Guzmán is supported by a predoctoral grant from the Technical University of Madrid. Victoria Fernández is supported by a Ramón y Cajal contract (MINECO, Spain), cofinanced by the European Social Fund.
Home All Categories Science & Math Books Evolution Books Microbial Ultrastructure: The Use of the Electron Microscope. ISBN13: 9780122694509. Microbial Ultrastructure. by Roy Fuller and . Select Format: Hardcover.
Despite being an excellent tool for investigating ultrastructure, scanning electron microscopy. SEM) is less frequently used than transmission electron microscopy for microbes such as viruses or. bacteria. ventional protocol involved dehydration in ethanol series, followed by air drying and metal coating before. For the ionic liquid protocol, the biological sample had a drop of a . % aqueous solution of. azolium tetrauoroborate placed directly on it. Aer blotting to remove excess uid the. wet sample was then placed directly in the SEM.
Electron microscopes are used to investigate the ultrastructure of a wide range of biological and inorganic specimens including microorganisms, cells, large molecules, biopsy samples, metals, and crystals. Modern electron microscopes produce electron micrographs using specialized digital cameras and frame grabbers to capture the images. One major disadvantage of the transmission electron microscope is the need for extremely thin sections of the specimens, typically about 100 nanometers. Creating these thin sections for biological and materials specimens is technically very challenging. Semiconductor thin sections can be made using a focused ion beam.