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Download DNA Transfer to Cultured Cells (Culture of Specialized Cells) epub book
ISBN:0471165727
Author: Katya Ravid
ISBN13: 978-0471165729
Title: DNA Transfer to Cultured Cells (Culture of Specialized Cells)
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ePUB size: 1629 kb
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Language: English
Category: Medicine and Health Sciences
Publisher: John Wiley & Sons; 1 edition (August 14, 1998)
Pages: 316

DNA Transfer to Cultured Cells (Culture of Specialized Cells) by Katya Ravid



Culture of Specialized Cells. Culture of hematopoietic cells. Culture of epithelial cells. tions between adjacent cells allowing the transfer of signal trans-ducers such as cyclic AMP, partly to the decrease in cell prolif-eration at high cell density, and partly to the shape change achieved as a result of crowding at high cell densities, as dis-cussed below (see Section . Organogenesis in the embryo occurs by the interaction of de-veloping epithelium from the endoderm or ectoderm with adjacent mesenchymal cells.

Библиографические данные. DNA Transfer to Cultured Cells Culture of Specialized Cells (Том 5). Автор.

Isolation and Culture of Vascular Endothelial Cells, 419 2. Neuroectodermal Cells, 422 2. Neurons, 422 Protocol 2. 9. Basic Cell Culture Techniques, 543 Exercise 7 Observation of Cultured Cells, 543 Exercise 8 Preparing Sterile Medium for Use, 545 Exercise 9 Feeding a Monolayer Culture, 546 Exercise 10 Preparation of Complete Medium from 10 Stock, 547 Exercise 11 Counting Cells by Hemocytometer and Electronic Counter, 548 Exercise 12 Subculture of Cells Growing in Suspension, 551 Exercise 13 Subculture of Cell Lines. Growing in Monolayer, 552 Exercise 14 Staining a Monolayer Cell Culture with Giemsa, 554 Exercise 15 Construction and Analysis of Growth Curve, 556 2.

Culture of specialized cells. General Note: Includes index. On this site it is impossible to download the book, read the book online or get the contents of a book. The administration of the site is not responsible for the content of the site. The data of catalog based on open source database. All rights are reserved by their owners. John Wiley & Sons. Different cell types require different schemes for DNA transfer; the book will describe these diverse methods, their application to specific cell types and their relevance to a biological problem. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. Partial table of contents: Production and Use of Retroviruses (G. Hicks, et a. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including: Viral infection Electroporation Phosphate precipitation DEAE Dextran Liposomes Yeast artificial chromosomes (YACs) Whole chromosome transfer Enhanced expression. Special sections at the end of each chapter list suppliers for necessary reagents and materials. DNA transfer to cultured cells. Scientists now have the means to introduce copies of genes intodifferent cell types, then detect the expression of these genes inthe cell.

Gene transfer and expression in cultured mammalian cells comprise a complex of techniques to introduce and express novel genetic material in a variety of eukaryotic cell types in an effort to examine the possible effect of its activity on intracellular processes. Keywords: gene transfer; transfection; cDNA; expression vector.

Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. DNA Transfer to Cultured Cells is the first quick reference to all of the established.

Transfection Non-viral DNA delivery RNA delivery siRNA delivery Nucleic acid transfer Knockdown Reporter assay. Springer Nature is developing a new tool to find and evaluate Protocols. 2005) Delivery of small interfering RNA to mammalian cells in culture by using cationic lipid/polymer-based transfection reagents. Cite this protocol as: Gopalakrishnan . Wolff J. (2009) siRNA and DNA Transfer to Cultured Cells. In: Belting M. (eds) Macromolecular Drug Delivery. Methods in Molecular Biology (Methods and Protocols), vol 480.

2 Biology of Cultured Cells. 19. 3 Laboratory Design and Layout.

DNA transfer to cultured cellsEdited by Katya Ravid and R. Ian FreshneyRapid advances in DNA transfer technology have transformed manydisciplines, ranging from molecular genetics to biotechnology.Scientists now have the means to introduce copies of genes intodifferent cell types, then detect the expression of these genes inthe cell. It is now possible to regulate cell growth that may leadto cancer, develop new biopharmaceuticals, and apply knowledgeabout the role of genes in cell processes to basic research inmolecular genetics.DNA Transfer to Cultured Cells is the first quick reference to allof the established techniques for the transfer of genetic materialto cells in vitro. Featuring contributions by leading researchersin the field, this detailed guide walks the reader through avariety of DNA transfer methods, describes their application tospecific cell types, and integrates aspects of molecular biologywith tissue culture. Offering overviews and detailed protocols forthe techniques under discussion in each of its sections, this bookcovers an exceptionally broad array of topics, including:* Viral infection* Electroporation* Phosphate precipitation* DEAE Dextran* Liposomes* Yeast artificial chromosomes (YACs)* Whole chromosome transfer* Enhanced expression.Special sections at the end of each chapter list suppliers fornecessary reagents and materials. This easy-to-use, self-containedguide addresses key developments of recent years as well asemerging trends in DNA transfer. For practical applications in cellbiology, genetics, heredity, biotechnology, or evolution, DNATransfer to Cultured Cells is a unique and unparalleled resource.