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ISBN:0896032892
Author: John R. Gosden
ISBN13: 978-0896032897
Title: Chromosome Analysis Protocols (Methods in Molecular Biology)
Format: rtf mbr lit lrf
ePUB size: 1402 kb
FB2 size: 1149 kb
DJVU size: 1124 kb
Language: English
Category: Medicine and Health Sciences
Publisher: Humana Press; 1994 edition (February 7, 1994)
Pages: 509

Chromosome Analysis Protocols (Methods in Molecular Biology) by John R. Gosden



Methods in Molecular Biology.

The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. My Blog! - Download NitroFlare.

Chromosome Analysis Protocols. This was the require­ ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series. Provides a ready source of both old and new cytogenetic methods and contains a wealth of troubleshooting tips from experienced workers in the field. Show all. Table of contents (27 chapters).

Protein Chromatography: Methods and Protocols (Methods in Molecular Biology 681)Documents. Macromolecular Crystallography Protocols Volume 363 Protein. Antibody Methods and Protocols Volume 901 Documents.

Written in the highly successful Methods in Molecular Biology™ series format, chapters include a brief introduction to their respective subjects, lists of the necessary materials and reagents, step-by-step protocols, and time-saving notes on troubleshooting and avoiding known pitfalls

Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The chapters Automatic Karyotyping Analysis by J. Graham and J. Piper, Oligonucleotide Primed in Situ DNA Synthesis (PRINS) by . Gosden and D. Lawson in addition to . Cookes chapter on Chromosome Substructure In-. vestigation provide interesting and knowledgeable in- sights into these areas of investigation. As a litmus test, I selected one chapter to evaluate if there was enough information to perform the technique using the materials and methods outlined. I picked Chapter 23, Chromosome Dissection and Cloning by .

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves­ tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports―as a result of pressure on space from editors, and the response to that pressure by authors―contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en­ countered in handling any biological system. This was the require­ ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.